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Correlation between the E test and the CLSI M-38 A microdilution method to determine the activity of amphotericin B, voriconazole, and itraconazole against clinical isolates of Aspergillus fumigatus
dc.contributor.author | Guinea, Jesus | |
dc.contributor.author | Pelaez, Teresa | |
dc.contributor.author | Alcala, Luis | |
dc.contributor.author | Bouza, Emilio | |
dc.date.accessioned | 2024-02-06T11:29:57Z | |
dc.date.available | 2024-02-06T11:29:57Z | |
dc.date.issued | 2007-03-01 | |
dc.identifier.citation | Guinea, J., Peláez, T., Alcalá, L., & Bouza, E. (2007). Correlation between the E test and the CLSI M-38 A microdilution method to determine the activity of amphotericin B, voriconazole, and itraconazole against clinical isolates of Aspergillus fumigatus. Diagnostic microbiology and infectious disease, 57(3), 273–276. https://doi.org/10.1016/j.diagmicrobio.2006.09.003 | es |
dc.identifier.issn | 0732-8893 | |
dc.identifier.other | http://www.journals.elsevier.com/diagnostic-microbiology-and-infectious-disease/ | es |
dc.identifier.other | https://pubmed.ncbi.nlm.nih.gov/17141455/ | es |
dc.identifier.uri | http://hdl.handle.net/20.500.12020/1245 | |
dc.description.abstract | The in vitro activities of amphotericin B, itraconazole, and voriconazole against 283 clinical isolates of Aspergillus fumigatus were studied by comparing the E test method with the reference procedure NCCLS (CLSI) M-38A. The methods were considered to agree when the results of the MICs by E test were within 2 dilutions of the MICs obtained by CLSI M-38 A. Agreement of readings at 24, 48, and 72 h of incubation was as follows: amphotericin B: 4.2%, 98%, and 40.6%; itraconazole: 92.5%, 100%, and 89.9%; and voriconazole: 98.9%, 100%, and 99.7%. The correlation between methods to classify the strains as susceptible or resistant was very good for itraconazole and voriconazole after 48 h of incubation, but 23% of the strains were incorrectly classified by E test for amphotericin B. The E test is an efficacious method for antifungal susceptibility testing in A. fumigatus for itraconazole and voriconazole when the plates are read after 48 h of incubation. The use of the E test to study the activity of amphotericin B should be avoided. | es |
dc.language.iso | en | es |
dc.publisher | ELSEVIER SCIENCE INC | es |
dc.title | Correlation between the E test and the CLSI M-38 A microdilution method to determine the activity of amphotericin B, voriconazole, and itraconazole against clinical isolates of Aspergillus fumigatus | es |
dc.type | article | es |
dc.identifier.doi | 10.1016/j.diagmicrobio.2006.09.003 | |
dc.issue.number | 3 | es |
dc.journal.title | Diagnostic Microbiology and Infectious Diseases | es |
dc.page.initial | 273 | es |
dc.page.final | 276 | es |
dc.relation.projectID | This study was partially financed by grant number C/03/ 14 from Red Espan˜ola de Investigacio´n en Patologı´a Infecciosa (REIPI, Instituto de Salud Carlos III, Madrid, Spain) and by grant number 08.2/0026/ 2001 1 from the Comunidad de Madrid Research Fund (FIS, Instituto de Salud Carlos III, Madrid, Spain). | es |
dc.rights.accessRights | embargoedAccess | es |
dc.subject.area | Ciencias Biomédicas | es |
dc.subject.keyword | Invasive aspergillosis | es |
dc.subject.keyword | Susceptibility | es |
dc.subject.keyword | E-test | es |
dc.subject.keyword | Posaconazole | es |
dc.subject.keyword | Resistance | es |
dc.subject.keyword | MICs | es |
dc.subject.unesco | 3201.03 Microbiología Clínica | es |
dc.volume.number | 57 | es |
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