Fingerprinting, embryo type and geographic differentiation in mango (Mangifera indica L., Anacardiaceae) with microsatellites
Identificadores
URI: http://hdl.handle.net/20.500.12020/1229ISSN: 1380-3743
DOI: 10.1007/s11032-004-7982-x
Autor/es
Viruel, María Ángeles; Escribano, Pilar; Barbieri, Mirko; Ferri, Marco; Hormaza, José IgnacioFecha
2005-01-01Tipo de documento
articleÁrea/s de conocimiento
Biología Celular y MolecularMateria/s Unesco
2415.02 Biología Molecular de PlantasResumen
We report the sequence and variability parameters of 16 microsatellite primer pairs obtained from two
mango (Mangifera indica L.) genomic libraries after digestion of DNA of the cultivar Tommy Atkins with
HaeIII and RsaI and enrichment in CT repeats. Although no significant differences were recorded between
the two libraries in the informativeness of the markers obtained, the RsaI library was shown to be more
useful than the HaeIII taking into account the efficiency of the library and the feasibility of clone
sequencing. The polymorphism revealed by those microsatellites was evaluated in a collection of 28 mango
cultivars of different origins. A total of 88 fragments were detected with the 16 simple sequence repeats
(SSRs) with an average of 5.5 bands/SSR. Two primer pairs amplified more than a single locus. The mean
expected and observed heterozygosities over the 14 single-locus SSRs averaged 0.65 and 0.69 respectively.
The total value for the probability of identity was 2.74 · 10 9. The SSRs studied allowed the unambiguous
identification of all the mango genotypes studied and this discrimination can be carried out with just three
selected microsatellites. UPGMA cluster analysis and Principal coordinates analysis group the genotypes
according to their origin and their classification as monoembryonic or polyembryonic types reflecting the
pedigree of the cultivars and the movement of mango germplasm. The results demonstrate the usefulness of
microsatellites for studies on identification, variability, germplasm conservation, domestication and
movement of germplasm in mango.