Comparison of different methods to construct a core germplasm collection in woody perennial species with simple sequence repeat markers. A case study in cherimoya (Annona cherimola, Annonaceae), an underutilised subtropical fruit tree species
Identificadores
URI: http://hdl.handle.net/20.500.12020/1225ISSN: 0003-4746
DOI: 10.1111/j.1744-7348.2008.00232.x
Fecha
2008-01-01Tipo de documento
articleÁrea/s de conocimiento
Biología Celular y MolecularMateria/s Unesco
2414 MicrobiologíaResumen
Although molecular markers are becoming the tool of choice to develop core
collections in plants, the examples of their use in woody perennial species are
very scarce. In this work, we used simple sequence repeat (SSR) marker data to
develop a core collection in an underutilised subtropical fruit tree species, cherimoya
(Annona cherimola, Annonaceae), from an initial collection of 279 genotypes
from different countries. We compared six alternative allocation
methods to construct the core collection, four not based upon the similarity
dendrogram [random sampling, maximisation strategy (M strategy) and simulated
annealing algorithm maximising both genetic diversity and number of
SSR alleles] and two based on dendrogram data (logarithmic strategy and
stepwise clustering). The diversity maintained in each subset was compared
with that present in the entire collection. The results obtained indicate that
the use of SSRs together with the M strategy is the most efficient method to
develop a core collection in cherimoya. In the best subset, with 40 accessions,
all the SSR alleles present in the whole collection were recovered and no significant
differences in frequency distribution of alleles for any of the loci studied
or in variability parameters (HO, HE) were recorded between the core and
the whole collection.