Chronic treatment with a dopamine uptake blocker changes dopamine and acetylcholine but not glutamate and GABA concentrations in prefrontal cortex, striatum and nucleus accumbens of the awake rat.
Identificadores
URI: http://hdl.handle.net/20.500.12020/1223ISSN: 0197-0186
DOI: 10.1016/j.neuint.2007.08.005
Fecha
2008Tipo de documento
articleÁrea/s de conocimiento
Ciencias BiomédicasResumen
The present study was aimed to investigate the effects of a chronic treatment with the dopamine uptake blocker nomifensine on the in vivo
extracellular concentrations of dopamine, acetylcholine, glutamate and GABA in the prefrontal cortex, striatum and nucleus accumbens. Male
Wistar rats received intraperitoneal (i.p.) daily injections of nomifensine (10 mg/kg) or saline for 22 days. Microdialysis experiments were
performed on days 1, 8, 15 and 22 of treatment to evaluate the effects of the injection of nomifensine or saline. Motor activity of the animals was
monitored during microdialysis experiments. Injections of nomifensine increased extracellular concentration of dopamine in striatum and nucleus
accumbens, but not in prefrontal cortex. Acetylcholine concentrations in striatum but not in nucleus accumbens were increased by nomifensine on
days 15 and 22 of treatment. In prefrontal cortex, nomifensine increased acetylcholine levels without differences among days. No changes were
found on glutamate and GABA concentrations in the three areas studied. Injections of nomifensine also increased spontaneous motor activity and
stereotyped behaviour without differences among days. These results show that systemic chronic treatment with a dopamine uptake blocker
produces differential effects on extracellular concentrations of dopamine and acetylcholine, but not glutamate and GABA, in different areas of the
brain.