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CD44 modulates cell migration and invasion in Ewing sarcoma cells
dc.contributor.author | Fernández-Tabanera, Enrique | |
dc.contributor.author | García-García, Laura | |
dc.contributor.author | Rodríguez-Martín, Carlos | |
dc.contributor.author | Cervera, Saint T. | |
dc.contributor.author | González-González, Laura | |
dc.contributor.author | Robledo, Cristina | |
dc.contributor.author | Josa, Santiago | |
dc.contributor.author | Chapado, Luis | |
dc.contributor.author | Monzón, Sara | |
dc.contributor.author | Martinez, Selene | |
dc.contributor.author | Melero Fernandez de Mera, Raquel Maria | |
dc.contributor.author | Alonso, Javier | |
dc.date.accessioned | 2024-02-05T18:37:18Z | |
dc.date.available | 2024-02-05T18:37:18Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Fernández-Tabanera E, García-García L, Rodríguez-Martín C, Cervera ST, González-González L, Robledo C, Josa S, Martínez S, Chapado L, Monzón S, Melero-Fernández de Mera RM, Alonso J. CD44 Modulates Cell Migration and Invasion in Ewing Sarcoma Cells. Int J Mol Sci. 2023 Jul 21;24(14):11774. doi: 10.3390/ijms241411774. PMID: 37511533; PMCID: PMC10381016. | es |
dc.identifier.issn | 14220067 | |
dc.identifier.other | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10381016/ | es |
dc.identifier.uri | http://hdl.handle.net/20.500.12020/1207 | |
dc.description.abstract | The chimeric EWSR1::FLI1 transcription factor is the main oncogenic event in Ewing sarcoma. Recently, it has been proposed that EWSR1::FLI1 levels can fluctuate in Ewing sarcoma cells, giving rise to two cell populations. EWSR1::FLI1low cells present a migratory and invasive phenotype, while EWSR1::FLI1high cells are more proliferative. In this work, we described how the CD44 standard isoform (CD44s), a transmembrane protein involved in cell adhesion and migration, is overexpressed in the EWSR1::FLI1low phenotype. The functional characterization of CD44s (proliferation, clonogenicity, migration, and invasion ability) was performed in three doxycycline-inducible Ewing sarcoma cell models (A673, MHH-ES1, and CADO-ES1). As a result, CD44s expression reduced cell proliferation in all the cell lines tested without affecting clonogenicity. Additionally, CD44s increased cell migration in A673 and MHH-ES1, without effects in CADO-ES1. As hyaluronan is the main ligand of CD44s, its effect on migration ability was also assessed, showing that high molecular weight hyaluronic acid (HMW-HA) blocked cell migration while low molecular weight hyaluronic acid (LMW-HA) increased it. Invasion ability was correlated with CD44 expression in A673 and MHH-ES1 cell lines. CD44s, upregulated upon EWSR1::FLI1 knockdown, regulates cell migration and invasion in Ewing sarcoma cells. | es |
dc.description.sponsorship | : This project was funded by Instituto de Salud Carlos III, grant numbers PI20CIII/00020, DTS18CIII/00005, Asociación Pablo Ugarte, grant numbers TRPV205/18; Asociación Candela Riera, Asociación Todos Somos Iván & Fundación Sonrisa de Alex, grant numbers TVP333-19, TVP-1324/15; ASION, grant number TVP141/17. Enrique Fernández-Tabanera is supported by Asociación Candela Riera, Asociación Todos Somos Iván & Fundación Sonrisa de Alex, Saint T. Cervera is supported by Asociación Pablo Ugarte and Raquel M. Melero is supported by a CIBERER contract. | es |
dc.language.iso | en | es |
dc.publisher | MDPI | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | CD44 modulates cell migration and invasion in Ewing sarcoma cells | es |
dc.type | article | es |
dc.identifier.doi | 10.3390/ijms241411774 | |
dc.issue.number | 11774 | es |
dc.journal.title | Frontiers in Oncology | es |
dc.page.initial | 1 | es |
dc.page.final | 24 | es |
dc.rights.accessRights | openAccess | es |
dc.subject.area | Biología Celular y Molecular | es |
dc.subject.area | Ciencias Biomédicas | es |
dc.subject.keyword | Ewing sarcoma | es |
dc.subject.keyword | CD44 | es |
dc.subject.keyword | EWSR1::FLI1 | es |
dc.subject.keyword | cell migration | es |
dc.subject.keyword | cell invasiveness | es |
dc.volume.number | 24 | es |