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The Transcription Factor FEZF1, a Direct Target of EWSR1-FLI1 in Ewing Sarcoma Cells, Regulates the Expression of Neural-Specific Genes
dc.contributor.author | García García, Laura | |
dc.contributor.author | Fernández Tabanera, Enrique | |
dc.contributor.author | Cervera, Saint T | |
dc.contributor.author | Melero Fernandez de Mera, Raquel Maria | |
dc.contributor.author | Josa, Santiago | |
dc.contributor.author | González-González, Laura | |
dc.contributor.author | Rodríguez-Martín, Carlos | |
dc.contributor.author | Grünewald, Thomas G P | |
dc.contributor.author | Alonso, Javier | |
dc.date.accessioned | 2024-02-05T18:34:48Z | |
dc.date.available | 2024-02-05T18:34:48Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | García-García L, Fernández-Tabanera E, Cervera ST, Melero-Fernández de Mera RM, Josa S, González-González L, Rodríguez-Martín C, Grünewald TGP, Alonso J. The Transcription Factor FEZF1, a Direct Target of EWSR1-FLI1 in Ewing Sarcoma Cells, Regulates the Expression of Neural-Specific Genes. Cancers (Basel). 2021 Nov 12;13(22):5668. doi: 10.3390/cancers13225668. PMID: 34830820; PMCID: PMC8616448. | es |
dc.identifier.issn | 2072-6694 | |
dc.identifier.other | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8616448/pdf/cancers-13-05668.pdf | es |
dc.identifier.uri | http://hdl.handle.net/20.500.12020/1205 | |
dc.description.abstract | Ewing sarcoma is a rare pediatric tumor characterized by chromosomal translocations that give rise to aberrant chimeric transcription factors (e.g., EWSR1-FLI1). EWSR1-FLI1 promotes a spe cific cellular transcriptional program. Therefore, the study of EWSR1-FLI1 target genes is important to identify critical pathways involved in Ewing sarcoma tumorigenesis. In this work, we focused on the transcription factors regulated by EWSR1-FLI1 in Ewing sarcoma. Transcriptomic analysis of the Ewing sarcoma cell line A673 indicated that one of the genes more strongly upregulated by EWSR1-FLI1 was FEZF1 (FEZ family zinc finger protein 1), a transcriptional repressor involved in neural cell identity. The functional characterization of FEZF1 was performed in three Ewing sarcoma cell lines (A673, SK-N-MC, SK-ES-1) through an shRNA-directed silencing approach. FEZF1 knock down inhibited clonogenicity and cell proliferation. Finally, the analysis of the FEZF1-dependent expression profile in A673 cells showed several neural genes regulated by FEZF1 and concomitantly regulated by EWSR1-FLI1. In summary, FEZF1 is transcriptionally regulated by EWSR1-FLI1 in Ewing sarcoma cells and is involved in the regulation of neural-specific genes, which could explain the neural-like phenotype observed in several Ewing sarcoma tumors and cell lines | es |
dc.language.iso | en | es |
dc.publisher | MDPI | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | The Transcription Factor FEZF1, a Direct Target of EWSR1-FLI1 in Ewing Sarcoma Cells, Regulates the Expression of Neural-Specific Genes | es |
dc.type | article | es |
dc.identifier.doi | 10.3390/CANCERS13225668 | |
dc.journal.title | Cancers | es |
dc.page.initial | 13 | es |
dc.page.final | 22 | es |
dc.rights.accessRights | openAccess | es |
dc.subject.area | Biología Celular y Molecular | es |
dc.subject.area | Ciencias Biomédicas | es |
dc.subject.keyword | Ewing sarcoma | es |
dc.subject.keyword | EWSR1-FLI1 | es |
dc.subject.keyword | GGAA-microsatellites | es |
dc.subject.keyword | FEZF11 | es |
dc.volume.number | 5668 | es |